Genotyping and primer details


Genotyping and primer details for 130H02 - At3g12160
Line ID 130H02
Locus At3g12160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3880475 of line 130H02 Chr3:3880475
   
Insertion allele amplicon 8474 - FK09
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FK09: 5'-CAATCAAATGGATGAATTGTGAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3880115
   
Insertion allele amplicon 3144 - ML23
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer ML23: 5'-AAATTGAACGGTGAATAAGGTACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3880781
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11