Genotyping and primer details


Genotyping and primer details for 754H06 - At3g13674
Line ID 754H06
Locus At3g13674 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4475779 of line 754H06 Chr3:4475779
   
Insertion allele amplicon 8474 - 01ux
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01ux: 5'-ATCTCTTCCTGAATCACACTCGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4476378
   
wt amplicon (over InsPos) 76vl - 01ux
wt amplicon start Chr3:4475317
wt amplicon end Chr3:4476378
wt amplicon size 1062 bp
Locus specific primer 01ux: 5'-ATCTCTTCCTGAATCACACTCGAT-3'
2nd locus specific primer 76vl: 5'-GGATACAAATTGCAACACAGAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11