Genotyping and primer details


Genotyping and primer details for 355F04 - At3g16320
Line ID 355F04
Locus At3g16320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5533367 of line 355F04 Chr3:5533367
   
Insertion allele amplicon 8474 - 18fp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18fp: 5'-TTATGCTTTGGAAGGAATGGATAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5532753
   
wt amplicon (over InsPos) 01gs - 18fp
wt amplicon start Chr3:5532753
wt amplicon end Chr3:5533755
wt amplicon size 1003 bp
Locus specific primer 18fp: 5'-TTATGCTTTGGAAGGAATGGATAC-3'
2nd locus specific primer 01gs: 5'-TTGTTGATGTGAAGAGCGTATTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11