Genotyping and primer details


Genotyping and primer details for 178D01 - At3g18610
Line ID 178D01
Locus At3g18610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6406279 of line 178D01 Chr3:6406279
   
Insertion allele amplicon 8474 - BU51
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BU51: 5'-GTATGGAGTTTTTACCTGCTTTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6405656
   
wt amplicon (over InsPos) 29si - BU51
wt amplicon start Chr3:6405656
wt amplicon end Chr3:6406783
wt amplicon size 1128 bp
Locus specific primer BU51: 5'-GTATGGAGTTTTTACCTGCTTTGC-3'
2nd locus specific primer 29si: 5'-GCAGTCCTTGAGAAGGCTAAGATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11