Genotyping and primer details


Genotyping and primer details for 435B04 - At3g46350
Line ID 435B04
Locus At3g46350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:17037285 of line 435B04 Chr3:17037285
   
Insertion allele amplicon 8474 - EQ69
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ69: 5'-TCTAAGGCCGATATAAATGGAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17037795
   
wt amplicon (over InsPos) KU45 - EQ69
wt amplicon start Chr3:17036718
wt amplicon end Chr3:17037795
wt amplicon size 1078 bp
Locus specific primer EQ69: 5'-TCTAAGGCCGATATAAATGGAATG-3'
2nd locus specific primer KU45: 5'-TCCTCGAAACGAACACTAATTTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11