Genotyping and primer details


Genotyping and primer details for 432E01 - At3g48400
Line ID 432E01
Locus At3g48400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:17925559 of line 432E01 Chr3:17925559
   
Insertion allele amplicon 3144 - 40yf
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 40yf: 5'-CTGATGGGTTAGGACTAGGACCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17926717
   
Insertion allele amplicon 8474 - NU13
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NU13: 5'-GTGTTCAACAACAAACCATTTACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17924974
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11