Genotyping and primer details


Genotyping and primer details for 180C10 - At3g53680
Line ID 180C10
Locus At3g53680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:19894116 of line 180C10 Chr3:19894116
   
Insertion allele amplicon 8474 - 10gp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10gp: 5'-AAACATGTTGGAAGAATTACCAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19893591
   
wt amplicon (over InsPos) 03cu - 10gp
wt amplicon start Chr3:19893591
wt amplicon end Chr3:19894714
wt amplicon size 1123 bp
Locus specific primer 10gp: 5'-AAACATGTTGGAAGAATTACCAGG-3'
2nd locus specific primer 03cu: 5'-TCTAAGGTTGTTCTCCGGTTCTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11