Genotyping and primer details


Genotyping and primer details for SALK_076686c - At3g55840
Line ID SALK_076686c
Locus At3g55840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20717916 of line SALK_076686c Chr3:20717916
   
Insertion allele amplicon R204 - BN51
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BN51: 5'-GCTACATAGCCTCAAGAACAATCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20718223
   
Insertion allele amplicon R212 - BN52
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer BN52: 5'-TTGTGTCAATATCTTCCATTGCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20717507
   
wt amplicon (over InsPos) BN51 - BN52
wt amplicon start Chr3:20717507
wt amplicon end Chr3:20718223
wt amplicon size 717 bp
Locus specific primer BN52: 5'-TTGTGTCAATATCTTCCATTGCAT-3'
2nd locus specific primer BN51: 5'-GCTACATAGCCTCAAGAACAATCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11