Genotyping and primer details


Genotyping and primer details for 562A03 - At3g58450
Line ID 562A03
Locus At3g58450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21621973 of line 562A03 Chr3:21621973
   
Insertion allele amplicon 8474 - 21eq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21eq: 5'-GTACTCTCTTCTCGTGCTTTCCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21622467
   
wt amplicon (over InsPos) 01fl - 21eq
wt amplicon start Chr3:21621718
wt amplicon end Chr3:21622467
wt amplicon size 750 bp
Locus specific primer 21eq: 5'-GTACTCTCTTCTCGTGCTTTCCTC-3'
2nd locus specific primer 01fl: 5'-TTTTGGGTTTATCTTTTTCTGAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11