Genotyping and primer details


Genotyping and primer details for 450D05 - At3g59020
Line ID 450D05
Locus At3g59020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21815031 of line 450D05 Chr3:21815031
   
Insertion allele amplicon 8474 - EP70
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EP70: 5'-CTAAACATTTTGGAACGTCCTGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21815530
   
wt amplicon (over InsPos) 19em - EP70
wt amplicon start Chr3:21814436
wt amplicon end Chr3:21815530
wt amplicon size 1095 bp
Locus specific primer EP70: 5'-CTAAACATTTTGGAACGTCCTGTT-3'
2nd locus specific primer 19em: 5'-AACCTCTTAAAAATGTCCACAACG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11