Genotyping and primer details


Genotyping and primer details for 191G09 - At3g61360
Line ID 191G09
Locus At3g61360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22706151 of line 191G09 Chr3:22706151
   
Insertion allele amplicon 8474 - CE03
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CE03: 5'-GTATGCTCATGGACTTGAAACTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22705694
   
Insertion allele amplicon 3144 - CE04
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer CE04: 5'-GCACACATAAAGCAAAAATCTCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22706715
   
wt amplicon (over InsPos) CE03 - CE04
wt amplicon start Chr3:22705694
wt amplicon end Chr3:22706715
wt amplicon size 1022 bp
Locus specific primer CE04: 5'-GCACACATAAAGCAAAAATCTCAA-3'
2nd locus specific primer CE03: 5'-GTATGCTCATGGACTTGAAACTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11