Genotyping and primer details


Genotyping and primer details for 009E06 - At4g00840
Line ID 009E06
Locus At4g00840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:355636 of line 009E06 Chr4:355636
   
Insertion allele amplicon 8474 - BG63
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BG63: 5'-TCACTGGGTCTCATCACATAACAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:355275
   
Insertion allele amplicon 3144 - CB42
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer CB42: 5'-TGTTGGATGTTATTGTCTTGCTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:356135
   
wt amplicon (over InsPos) CB42 - BG63
wt amplicon start Chr4:355275
wt amplicon end Chr4:356135
wt amplicon size 861 bp
Locus specific primer BG63: 5'-TCACTGGGTCTCATCACATAACAT-3'
2nd locus specific primer CB42: 5'-TGTTGGATGTTATTGTCTTGCTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11