Genotyping and primer details


Genotyping and primer details for 107G03 - At4g04570
Line ID 107G03
Locus At4g04570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:2292558 of line 107G03 Chr4:2292558
   
Insertion allele amplicon 8474 - CD48
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CD48: 5'-AGAAGGACTTGCAGCTTTTGTAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:2261669
   
Insertion allele amplicon 3144 - CD49
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer CD49: 5'-CGAATCCAAGATTATGCGATTTAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:2292812
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11