Genotyping and primer details


Genotyping and primer details for 306D02 - At4g29730
Line ID 306D02
Locus At4g29730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14559440 of line 306D02 Chr4:14559440
   
Insertion allele amplicon 8474 - 14wj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14wj: 5'-AGCACTTGAGATTTGGATTTCACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14558932
   
wt amplicon (over InsPos) 14xd - 14wj
wt amplicon start Chr4:14558932
wt amplicon end Chr4:14560327
wt amplicon size 1396 bp
Locus specific primer 14wj: 5'-AGCACTTGAGATTTGGATTTCACT-3'
2nd locus specific primer 14xd: 5'-TTTTAGCCAGATCGACATTTGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11