Genotyping and primer details


Genotyping and primer details for 468C04 - At4g31700
Line ID 468C04
Locus At4g31700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15346992 of line 468C04 Chr4:15346992
   
Insertion allele amplicon 8474 - 30kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 30kh: 5'-TTTTGCTTCTCTGTTTCATCTTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15347551
   
wt amplicon (over InsPos) 03le - 30kh
wt amplicon start Chr4:15346447
wt amplicon end Chr4:15347551
wt amplicon size 1105 bp
Locus specific primer 30kh: 5'-TTTTGCTTCTCTGTTTCATCTTCA-3'
2nd locus specific primer 03le: 5'-AGCAATTTTCTTCTTCTTGTCAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11