Genotyping and primer details


Genotyping and primer details for 763E09 - At4g35920
Line ID 763E09
Locus At4g35920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17012317 of line 763E09 Chr4:17012317
   
Insertion allele amplicon 8474 - BK79
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BK79: 5'-TCTGCAAAGTTCTCAAAGATTCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17012913
   
wt amplicon (over InsPos) 12tf - BK79
wt amplicon start Chr4:17012070
wt amplicon end Chr4:17012913
wt amplicon size 844 bp
Locus specific primer BK79: 5'-TCTGCAAAGTTCTCAAAGATTCAA-3'
2nd locus specific primer 12tf: 5'-CGTTGTTTTTGTTCACCTTTTGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11