Genotyping and primer details


Genotyping and primer details for 691E01 - At5g01550
Line ID 691E01
Locus At5g01550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:215228 of line 691E01 Chr5:215228
   
Insertion allele amplicon 8474 - 49wl
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 49wl: 5'-GTGCAAGAAGAAATGTATGTTGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:215857
   
Insertion allele amplicon 8474 - EU37
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU37: 5'-CAAATACCTAAGCACCGTTCTCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:214691
   
wt amplicon (over InsPos) EU38 - EU37
wt amplicon start Chr5:214691
wt amplicon end Chr5:215662
wt amplicon size 972 bp
Locus specific primer EU37: 5'-CAAATACCTAAGCACCGTTCTCAT-3'
2nd locus specific primer EU38: 5'-ATCGTAGCTCTATCGGGAGTAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11