Genotyping and primer details


Genotyping and primer details for SALK_148550c - At5g10270
Line ID SALK_148550c
Locus At5g10270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3224877 of line SALK_148550c Chr5:3224877
   
Insertion allele amplicon LBb3 - 28hg
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 28hg: 5'-GTAGATGCCACCCTTGTATTTGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3223831
   
wt amplicon (over InsPos) 19lt - 28hg
wt amplicon start Chr5:3223831
wt amplicon end Chr5:3224953
wt amplicon size 1123 bp
Locus specific primer 28hg: 5'-GTAGATGCCACCCTTGTATTTGTT-3'
2nd locus specific primer 19lt: 5'-TTTTGAAAACCGGTCAATAAAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11