Genotyping and primer details


Genotyping and primer details for 752D10 - At5g17440
Line ID 752D10
Locus At5g17440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5752912 of line 752D10 Chr5:5752912
   
Insertion allele amplicon 8474 - 60sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 60sm: 5'-TGGTTGGTAGTCCTCATTCTCTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5752394
   
wt amplicon (over InsPos) 27tc - 60sm
wt amplicon start Chr5:5752394
wt amplicon end Chr5:5753472
wt amplicon size 1079 bp
Locus specific primer 60sm: 5'-TGGTTGGTAGTCCTCATTCTCTCT-3'
2nd locus specific primer 27tc: 5'-TCCTAAACGCATCTGTAAGAACCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11