Genotyping and primer details


Genotyping and primer details for 121F07 - At5g23230
Line ID 121F07
Locus At5g23230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:7826265 of line 121F07 Chr5:7826265
   
Insertion allele amplicon 8474 - NB42
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NB42: 5'-TTATGCAATATGCATGGACGTACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7825590
   
Insertion allele amplicon 3144 - NB43
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer NB43: 5'-CTTTCGTTCCCAAATATATCTCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7826656
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11