Genotyping and primer details


Genotyping and primer details for 732G05 - At5g49680
Line ID 732G05
Locus At5g49680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20181266 of line 732G05 Chr5:20181266
   
Insertion allele amplicon 8474 - 70ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 70ra: 5'-GAAAGCTGTTGAGAGAAGAGGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20181611
   
wt amplicon (over InsPos) 25rt - 70ra
wt amplicon start Chr5:20181021
wt amplicon end Chr5:20181611
wt amplicon size 591 bp
Locus specific primer 70ra: 5'-GAAAGCTGTTGAGAGAAGAGGAAA-3'
2nd locus specific primer 25rt: 5'-GGCATTCGCAAGTTAATATTTGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11