Genotyping and primer details


Genotyping and primer details for 765H11 - At5g54690
Line ID 765H11
Locus At5g54690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22220735 of line 765H11 Chr5:22220735
   
Insertion allele amplicon 8474 - 20kn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 20kn: 5'-AAAGCCAAAGCTTTTAGGAAGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22221073
   
wt amplicon (over InsPos) 11li - 20kn
wt amplicon start Chr5:22220130
wt amplicon end Chr5:22221073
wt amplicon size 944 bp
Locus specific primer 20kn: 5'-AAAGCCAAAGCTTTTAGGAAGAAG-3'
2nd locus specific primer 11li: 5'-TTATAGGTTTCGGGTCTAATGCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11