Genotyping and primer details


Genotyping and primer details for 007C10 - At3g27810
Line ID 007C10
Locus At3g27810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:10308869 of line 007C10 Chr3:10308869
   
Insertion allele amplicon 8474 - DR06
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DR06: 5'-TTCAGTTGTGTCTGTCATGAGCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:10309526
   
Insertion allele amplicon 3144 - FM57
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FM57: 5'-CAAGACATTTGGTGACTAAAGCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:10308574
   
wt amplicon (over InsPos) DR06 - FM57
wt amplicon start Chr3:10308574
wt amplicon end Chr3:10309526
wt amplicon size 953 bp
Locus specific primer FM57: 5'-CAAGACATTTGGTGACTAAAGCAA-3'
2nd locus specific primer DR06: 5'-TTCAGTTGTGTCTGTCATGAGCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11