Genotyping and primer details


Genotyping and primer details for 022D04 - At5g37180
Line ID 022D04
Locus At5g37180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:14719897 of line 022D04 Chr5:14719897
   
Insertion allele amplicon 8474 - FL33
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FL33: 5'-TGAGCATCAAGGAGAGGTTAGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:14719415
   
Insertion allele amplicon 8474 - MS12
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MS12: 5'-TTGGTCAAGAATGTAAACGACTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:14720433
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11