Genotyping and primer details


Genotyping and primer details for 052H10 - T24P13
Line ID 052H10
Locus T24P13 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:9277193 of line 052H10 Chr1:9277193
   
Insertion allele amplicon 3144 - MZ82
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer MZ82: 5'-ACATCTTTCCTGTGCTACTCCAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:9277730
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11