Genotyping and primer details

Genotyping and primer details for 058D11 - At4g11560
Line ID 058D11
Locus At4g11560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:7001594 of line 058D11 Chr4:7001594
Insertion allele amplicon 8474 - MK65
Locus specific primer MK65: 5'-AAAATACTTGGAAGAAACGCTGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:7001043
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11