Genotyping and primer details


Genotyping and primer details for 064B11 - At4g20140
Line ID 064B11
Locus At4g20140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10887853 of line 064B11 Chr4:10887853
   
Insertion allele amplicon 8474 - AB77
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AB77: 5'-CGGGATTAATTTTGCGTGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10888537
   
wt amplicon (over InsPos) 06ft - AB77
wt amplicon start Chr4:10887429
wt amplicon end Chr4:10888537
wt amplicon size 1109 bp
Locus specific primer AB77: 5'-CGGGATTAATTTTGCGTGAT-3'
2nd locus specific primer 06ft: 5'-GTACCTTCATCATGACTGTGTTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11