Genotyping and primer details


Genotyping and primer details for 073A06 - At4g30930
Line ID 073A06
Locus At4g30930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15050402 of line 073A06 Chr4:15050402
   
Insertion allele amplicon 8474 - IS09
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IS09: 5'-TTTCTCTTTATGTCCCTTTGCAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15050898
   
wt amplicon (over InsPos) 17ax - IS09
wt amplicon start Chr4:15049942
wt amplicon end Chr4:15050898
wt amplicon size 957 bp
Locus specific primer IS09: 5'-TTTCTCTTTATGTCCCTTTGCAGT-3'
2nd locus specific primer 17ax: 5'-CAAGCATGAGCCAGTTTAAATACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11