Genotyping and primer details


Genotyping and primer details for 075G01 - At1g54970
Line ID 075G01
Locus At1g54970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20505002 of line 075G01 Chr1:20505002
   
Insertion allele amplicon 8474 - XZ01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer XZ01: 5'-ATATGAAAACAAACCAATCCGTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20504431
   
wt amplicon (over InsPos) 08dy - XZ01
wt amplicon start Chr1:20504431
wt amplicon end Chr1:20505471
wt amplicon size 1041 bp
Locus specific primer XZ01: 5'-ATATGAAAACAAACCAATCCGTCT-3'
2nd locus specific primer 08dy: 5'-AGAGACTTGGCTTATTGTTAACCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11