Genotyping and primer details

Genotyping and primer details for 089H05 - At4g03550
Line ID 089H05
Locus At4g03550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1575842 of line 089H05 Chr4:1575842
Insertion allele amplicon 8474 - VL20
Locus specific primer VL20: 5'-TTGACTTGACTGTACAAGCTGTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1575437
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11