Genotyping and primer details


Genotyping and primer details for 091D11 - At4g08920
Line ID 091D11
Locus At4g08920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:5726286 of line 091D11 Chr4:5726286
   
Insertion allele amplicon 8474 - MR42
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MR42: 5'-AAATTTCATGCCACTTGGTTAGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:5726877
   
wt amplicon (over InsPos) 01lw - MR42
wt amplicon start Chr4:5725831
wt amplicon end Chr4:5726877
wt amplicon size 1047 bp
Locus specific primer MR42: 5'-AAATTTCATGCCACTTGGTTAGAC-3'
2nd locus specific primer 01lw: 5'-CTAGACTCCCGACAGACTGGATAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11