Genotyping and primer details


Genotyping and primer details for 096A10 - At1g02170
Line ID 096A10
Locus At1g02170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:412261 of line 096A10 Chr1:412261
   
Insertion allele amplicon 8474 - PP02
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PP02: 5'-GGTAAATCCAGAACGGTACCACTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:412769
   
wt amplicon (over InsPos) 04cx - PP02
wt amplicon start Chr1:411693
wt amplicon end Chr1:412769
wt amplicon size 1077 bp
Locus specific primer PP02: 5'-GGTAAATCCAGAACGGTACCACTA-3'
2nd locus specific primer 04cx: 5'-TGGGCTTAAAAACTGTTATATGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11