Genotyping and primer details


Genotyping and primer details for 098D07 - At2g37170
Line ID 098D07
Locus At2g37170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15614682 of line 098D07 Chr2:15614682
   
Insertion allele amplicon 8474 - DU05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DU05: 5'-GAAGACTGTGTAGACGAGAACGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15614119
   
Insertion allele amplicon 3144 - IW28
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer IW28: 5'-CATAAGCCTACCAAAAGGAAAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15615009
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11