Genotyping and primer details


Genotyping and primer details for 106G06 - At5g54250
Line ID 106G06
Locus At5g54250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22029644 of line 106G06 Chr5:22029644
   
Insertion allele amplicon 8474 - RK25
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RK25: 5'-TATTATTTTTGGTCCACACACGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22028810
   
wt amplicon (over InsPos) 15fo - RK25
wt amplicon start Chr5:22028810
wt amplicon end Chr5:22030051
wt amplicon size 1242 bp
Locus specific primer RK25: 5'-TATTATTTTTGGTCCACACACGAA-3'
2nd locus specific primer 15fo: 5'-TTCATCTCCAAGATTTCTCTCCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11