Genotyping and primer details

Genotyping and primer details for 123D02 - At1g74890
Line ID 123D02
Locus At1g74890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28132935 of line 123D02 Chr1:28132935
Insertion allele amplicon 8474 - DL29
Locus specific primer DL29: 5'-AAACCAAACAAAAGAAAAGCAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28132527
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11