Genotyping and primer details


Genotyping and primer details for 123D07 - At3g18030
Line ID 123D07
Locus At3g18030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6168296 of line 123D07 Chr3:6168296
   
Insertion allele amplicon 8474 - IY01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IY01: 5'-AACAGTCGAACTCAGAATCGAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6168901
   
wt amplicon (over InsPos) 02ua - IY01
wt amplicon start Chr3:6167725
wt amplicon end Chr3:6168901
wt amplicon size 1177 bp
Locus specific primer IY01: 5'-AACAGTCGAACTCAGAATCGAAAT-3'
2nd locus specific primer 02ua: 5'-AGTGGAGCTAGTAGTGCAAATGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11