Genotyping and primer details


Genotyping and primer details for 131F04 - At3g07650
Line ID 131F04
Locus At3g07650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2443685 of line 131F04 Chr3:2443685
   
Insertion allele amplicon 8474 - GC01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GC01: 5'-TTTGGACAAGGAAAGTCAGAGAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2444158
   
wt amplicon (over InsPos) 13dn - GC01
wt amplicon start Chr3:2443279
wt amplicon end Chr3:2444158
wt amplicon size 880 bp
Locus specific primer GC01: 5'-TTTGGACAAGGAAAGTCAGAGAAT-3'
2nd locus specific primer 13dn: 5'-CAAACAGCTCCAGAGGTTTTTATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11