Genotyping and primer details


Genotyping and primer details for 136D10 - At2g42300
Line ID 136D10
Locus At2g42300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17623580 of line 136D10 Chr2:17623580
   
Insertion allele amplicon 8474 - H48a
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer H48a: 5'-CTGTGTTTTGGGGTCCATCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17622872
   
wt amplicon (over InsPos) 18hf - H48a
wt amplicon start Chr2:17622872
wt amplicon end Chr2:17623928
wt amplicon size 1057 bp
Locus specific primer H48a: 5'-CTGTGTTTTGGGGTCCATCT-3'
2nd locus specific primer 18hf: 5'-AGAATAGGGTCGATTAATGTTGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11