Genotyping and primer details


Genotyping and primer details for 166H11 - At2g15300
Line ID 166H11
Locus At2g15300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:6651486 of line 166H11 Chr2:6651486
   
Insertion allele amplicon 3144 - 58wt
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 58wt: 5'-ATGGATAAGCAGAAGGTACGAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:6650865
   
Insertion allele amplicon 8474 - NK34
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NK34: 5'-TGGGTTTGTAGGCTTTGAGAGTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:6652219
   
wt amplicon (over InsPos) MN60 - NK34
wt amplicon start Chr2:6651286
wt amplicon end Chr2:6652219
wt amplicon size 934 bp
Locus specific primer NK34: 5'-TGGGTTTGTAGGCTTTGAGAGTAT-3'
2nd locus specific primer MN60: 5'-GGAAACCTACCTCTATCCTCCATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11