Genotyping and primer details


Genotyping and primer details for 171B05 - At1g55530
Line ID 171B05
Locus At1g55530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20730270 of line 171B05 Chr1:20730270
   
Insertion allele amplicon 8474 - BD69
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BD69: 5'-ACTACTGCTTCCATTGTTATCGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20729664
   
wt amplicon (over InsPos) 04tf - BD69
wt amplicon start Chr1:20729664
wt amplicon end Chr1:20730735
wt amplicon size 1072 bp
Locus specific primer BD69: 5'-ACTACTGCTTCCATTGTTATCGCT-3'
2nd locus specific primer 04tf: 5'-GGGTTTTTCAGTGTCTTGATGATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 31.05.2018 12:55