Genotyping and primer details


Genotyping and primer details for 175F10 - At2g36350
Line ID 175F10
Locus At2g36350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15240777 of line 175F10 Chr2:15240777
   
Insertion allele amplicon 8474 - KP07
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KP07: 5'-GTTTCTTGCTTGGGTTGATAAGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15241306
   
wt amplicon (over InsPos) 05sq - KP07
wt amplicon start Chr2:15240349
wt amplicon end Chr2:15241306
wt amplicon size 958 bp
Locus specific primer KP07: 5'-GTTTCTTGCTTGGGTTGATAAGAG-3'
2nd locus specific primer 05sq: 5'-GGTGATGCAGATGAACAGATAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11