Genotyping and primer details

Genotyping and primer details for 181H08 - At5g60370
Line ID 181H08
Locus At5g60370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24283532 of line 181H08 Chr5:24283532
Insertion allele amplicon 8474 - 12cy
Locus specific primer 12cy: 5'-AGGACATTAACACAAAACTCCCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24284037
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11