Genotyping and primer details


Genotyping and primer details for 203C07 - At1g10930
Line ID 203C07
Locus At1g10930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3652446 of line 203C07 Chr1:3652446
   
Insertion allele amplicon 8474 - HP02
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HP02: 5'-TTGCTTGCAATATCAAATGAACTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3653028
   
wt amplicon (over InsPos) 06rd - HP02
wt amplicon start Chr1:3652105
wt amplicon end Chr1:3653028
wt amplicon size 924 bp
Locus specific primer HP02: 5'-TTGCTTGCAATATCAAATGAACTC-3'
2nd locus specific primer 06rd: 5'-TGATATGTCAAACTCTTCCCTCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11