Genotyping and primer details


Genotyping and primer details for 207D11 - At1g60220
Line ID 207D11
Locus At1g60220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:22210460 of line 207D11 Chr1:22210460
   
Insertion allele amplicon 8474 - GC09
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GC09: 5'-TACTGCAATTTTGTTGTGACATGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22209752
   
wt amplicon (over InsPos) 07cm - GC09
wt amplicon start Chr1:22209752
wt amplicon end Chr1:22210652
wt amplicon size 901 bp
Locus specific primer GC09: 5'-TACTGCAATTTTGTTGTGACATGA-3'
2nd locus specific primer 07cm: 5'-CATGCAAATAGCGCTAGCATAATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11