Genotyping and primer details


Genotyping and primer details for 216D08 - At4g15880
Line ID 216D08
Locus At4g15880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9014462 of line 216D08 Chr4:9014462
   
Insertion allele amplicon 8474 - AY11
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AY11: 5'-GGTCTTGGCTACTCATGAAAACTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9013983
   
wt amplicon (over InsPos) 03rd - AY11
wt amplicon start Chr4:9013983
wt amplicon end Chr4:9014929
wt amplicon size 947 bp
Locus specific primer AY11: 5'-GGTCTTGGCTACTCATGAAAACTC-3'
2nd locus specific primer 03rd: 5'-TTTTAATGCATCCAACATGTCAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11