Genotyping and primer details


Genotyping and primer details for 223B05 - At1g19360
Line ID 223B05
Locus At1g19360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6690938 of line 223B05 Chr1:6690938
   
Insertion allele amplicon 8474 - PB24
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PB24: 5'-TTATGTAAAGAGAACGATGTTGCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6691367
   
wt amplicon (over InsPos) 02cr - PB24
wt amplicon start Chr1:6690567
wt amplicon end Chr1:6691367
wt amplicon size 801 bp
Locus specific primer PB24: 5'-TTATGTAAAGAGAACGATGTTGCG-3'
2nd locus specific primer 02cr: 5'-AGAAGGCATGAAGCAGTAAACATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11