Genotyping and primer details


Genotyping and primer details for 228E10 - At5g41970
Line ID 228E10
Locus At5g41970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16792040 of line 228E10 Chr5:16792040
   
Insertion allele amplicon 8474 - NE17
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NE17: 5'-CTGAAGTAGCTCTGGCTTAGGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16791483
   
Insertion allele amplicon 8474 - NE18
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NE18: 5'-AAAAGGTGTACCTGGTAAATGACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16792667
   
wt amplicon (over InsPos) NE17 - NE18
wt amplicon start Chr5:16791483
wt amplicon end Chr5:16792667
wt amplicon size 1185 bp
Locus specific primer NE18: 5'-AAAAGGTGTACCTGGTAAATGACG-3'
2nd locus specific primer NE17: 5'-CTGAAGTAGCTCTGGCTTAGGAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11