Genotyping and primer details


Genotyping and primer details for 235B06 - At5g21170
Line ID 235B06
Locus At5g21170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:7206092 of line 235B06 Chr5:7206092
   
Insertion allele amplicon 8474 - CK63
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CK63: 5'-TGAGCGACCAGTAAATGACTAGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7206419
   
wt amplicon (over InsPos) 17dk - CK63
wt amplicon start Chr5:7205635
wt amplicon end Chr5:7206419
wt amplicon size 785 bp
Locus specific primer CK63: 5'-TGAGCGACCAGTAAATGACTAGAG-3'
2nd locus specific primer 17dk: 5'-CTTCACGTCTGGTTTTCTCTCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11