Genotyping and primer details


Genotyping and primer details for 235F11 - At4g17420
Line ID 235F11
Locus At4g17420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9723085 of line 235F11 Chr4:9723085
   
Insertion allele amplicon 8474 - PP40
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PP40: 5'-CTTTTTCTTGTTGTTGTGTAGCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9723461
   
wt amplicon (over InsPos) 08ft - PP40
wt amplicon start Chr4:9722764
wt amplicon end Chr4:9723461
wt amplicon size 698 bp
Locus specific primer PP40: 5'-CTTTTTCTTGTTGTTGTGTAGCCA-3'
2nd locus specific primer 08ft: 5'-AAACGGTAAACTTTGGATGAACAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11