Genotyping and primer details


Genotyping and primer details for 237E06 - At5g58460
Line ID 237E06
Locus At5g58460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23633886 of line 237E06 Chr5:23633886
   
Insertion allele amplicon 8474 - RK94
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RK94: 5'-GAATTGCGAATACAGAGAAAGGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23633359
   
wt amplicon (over InsPos) 12id - RK94
wt amplicon start Chr5:23633359
wt amplicon end Chr5:23634458
wt amplicon size 1100 bp
Locus specific primer RK94: 5'-GAATTGCGAATACAGAGAAAGGAC-3'
2nd locus specific primer 12id: 5'-TAAGTACATTGCTGCAGTTAGCGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11